TABLE 1.
Oligonucleotides employed for mutagenesis
| Name | Sequence (5′→3′)a |
|---|---|
| 196S F | GTTGTTTCAAAATCGCAAGAGTACAAAATTCAAATTTTTGG |
| 196S R | CGATTTTGAAACAACCATGATCCATTGATTTGAATCTTCATGC |
| 196E F | GTTGTTGAAAAATCGCAAGAGTACAAAATTCAAATTTTTGG |
| 196E R | CGATTTTTCAACAACCATGATCCATTGATTTGAATCTTCATGC |
| W47Y F | GAAAAAGGATATATGAATGATCGAATGGTCTATTTCTACGATAA |
| W47Y R | GATCATTCATATATCCTTTTTCCGGAGTAAAATGAATTAATGGCCGGTTATAC |
| N49S F | GGATGGATGTCAGATCCGAATGGTCTATTCTACGAT |
| N49S R | ATTCGGATCTGACATCCATCCTTTTTCCGGAGTAAA |
| N52S F | GAATGATCCGAGTGGTCTATTCTACGATAAAACTGCTAAGCTTTG |
| N52S R | GAATAGACCACTCGGATCATTCATCCATCCTTTTCCGGAGTAAATAC |
| S111T F | GGTATCTTTACTGGTAGTATTGTCGTTGACCATAATAATACCTCT |
| S111T R | AATACTACCAGTAAAGATACCTTCATTATCGTGTTCAGGTCCAAT |
| K181F F | CGTGATCCATTCGTTTTCTGGCATGAAGATTCAAAT |
| K181F R | CCAGAAAACGAATGGATCACGGAATTGGTTTGAGGA |
| P232V F | TATGAATGTGTCGGTTTAATTGAAGTTCCTATTGAGAATTCAGAC |
| P232V R | AATTAAACCGACACATTCATACTGATTTCCGTAATAACCAGAAGA |
a
The underlined nucleotides indicate the position of the altered codon.