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Comparative analysis of PGT activities. The maximal activity (100%) corresponds to complete glycosylation of 1 μM BH4 in the reaction mixture, which contained 0.5 mM UDP-xylose for CY-049 PGT or 0.5 mM UDP-glucose for the other PGTs. The mixtures were incubated for 10 min with the indicated amounts of proteins.
