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Surface assembly of a functional minicellulosome through intracellular complementation, using a synthetic yeast consortium. The basic design consisted of four different engineered yeast strains capable of either displaying a trifunctional scaffoldin Scaf-ctf (SC) or secreting one of the three corresponding dockerin-tagged enzymes (endoglucanase [AT], exoglucanase [EC/CB], or β-glucosidase [BF]).
