FIG. 2.

Secretion of dockerin-tagged enzymes. (A to D) Enzyme activities of different secretion strains in the growth medium were examined by using either CMC (A to C) or p-4-methylumbellifery-β-d-glucopyranoside (D) as the substrate. For the CMC assay, Congo red was added as an indicator. (E to G) Binding of secreted enzymes onto surface-displayed Scaf-ctf was confirmed by immunofluorescence microscopy. Cells were probed with anti-C-His₆ serum and fluorescently stained with a goat anti-mouse IgG conjugated with Alexa Fluor 488. The growth medium for EBY100/pScat-ctf was used as a control in all cases.