FIG. 3.

Cellulose hydrolysis and ethanol production by different synthetic consortia (see Table 2). Different consortia were established using a strain displaying Scaf-ctf (SC), a strain carrying pCEL15 (CE) as a control, an At-secreting strain (AT), an Ec-secreting strain (EC), a CHBc-secreting strain (CB), and/or a Bglf-secreting strain (BF). (A) Ethanol production from PASC by different synthetic consortia. (B) Docking of At, Ec, and/or BglF onto Sacf-ctf-displaying cells in different consortia. Cells were probed with either anti-C-myc or anti-C-His₆ serum and fluorescently stained with a goat anti-mouse IgG conjugated with Alexa Fluor 488. Whole-cell fluorescence was determined using a fluorescence microplate reader. The dark arrow and gray arrow indicate the increase in fluorescence intensity after At and CBHII docking. (C) Cellulose hydrolysis (dashed lines) and ethanol production (solid lines) from PASC by a consortium composed of SC, AT, CB, and BF.