FIG. 1.
Physiological characterization of E. coli K24K (A, C, and E) and K24KL (its ldhA derivative) (B, D, and F) in shaken-flask cultures in MYA medium supplemented with 30 g·liter−1 glycerol under aerobic conditions. (A and B) Growth, polymer synthesis, and redox ratios; (C and D) time course plots for the synthesis of relevant bioproducts; (E and F) intracellular content of reduced and oxidized nucleotides. The redox ratio was defined as the ratio of reduced to oxidized cofactors ([NADH + NADPH]/[NAD+ + NADP+]). Symbols and bars represent the mean values for each parameter ± standard deviations of duplicate measurements from at least three independent cultures. CDW, cell dry weight; concn, concentration.