TABLE 1.
Plasmids used in the study
| Plasmid | Resistancea | Description (source or reference) |
|---|---|---|
| pPBMB101 | Kanr | Plasmid with an E. coli lacZ gene, used as template to amplify lacZEc (8) |
| pCR8/GW/TOPO | Specr | Gateway PCR entry vector (Invitrogen) |
| pCR2.1/TOPO | Kanr, Ampr | PCR cloning vector (Invitrogen) |
| pBSV2G | Gentr | B. burgdorferi shuttle vector (13) |
| pBSV2G_dvB2 | Gentr | Altered pBSV2G, Gateway destination vector (J. A. Carroll, unpublished) |
| pBH-lacZEc | Gentr | E. coli lacZEc cloned into pBSV2G_dvB2 (this work) |
| pBHflaBp-lacZEc | Gentr | pBH-lacZEc with the flaB promoter cloned upstream of lacZEc (this work) |
| pBH-lacZBb | Gentr | B. burgdorferi lacZBb cloned into pBSV2G (this work) |
| pBHflaBp-lacZBb | Gentr | pBH-lacZBb with the flaB promoter cloned upstream of lacZBb (this work) |
| pBHospCp-lacZBb | Gentr | pBH-lacZBb with the ospC promoter cloned upstream of lacZBb (this work) |
a
Kan, kanamycin; Spec, spectinomycin; Amp, ampicillin; Gent, gentamicin.