TABLE 2.

Primers used in the study

Primer no.a	Name	Sequence (5′-3′)b
1	Long lacZ 5′ (NotI)	AAGGAAAAAAGCGGCCGCTCTAGAGGATCCCGGGTCGACTAGTCTGCAGCTCGAGT
2	Long lacZ 3′ (NotI)	AAGGAAAAAAGCGGCCGCAGATCTTACTCAGGAGAGCGTTCACCGACAAACAACAG
3	lacZ 430	TGATGAAAGCTGGCTACAGGAAGG
4	lacZ 908	GTCACACTACGTCTGAACGTCGAA
5	lacZ 1525	GGCCACCGATATTATTTGCCCGAT
6	lacZ 2053	GGATGTCGCTCCACAAGGTAAACA
7	lacZ 2593	TATCAGCCGGAAAACCTACCGGAT
8	lacZ 3121	GCTGTTTTGGCGGATGAGAGAAGA
9	lacZ 1533 5′	ATATTATTTGCCCGATGTACGCGCGCGTGGATGAA
10	lacZ 1538 3′	TTCATCCACGCGCGCGTACATCGGGCAAAT
11	flaB promoter 5′ (BamHI)	CCCGCGGATCCCTGTCGCCTCTTGTGGCTTC
12	flaB promoter 3′ (XhoI)	CCCCGCTCGAGCATATATCATTCCTCCATGA
13	ospC promoter 5′ (BamHI)	CCCGCGGATCCAATTAAAACTTTTTTTATTAAAGTA
14	ospC promoter 3′ (XhoI)	CCCCGCTCGAGTAATTTGTGCCTCCTTTTTATTTAT
15	flaB forward	TCTTTTCTCTGGTGAGGGAGCT
16	flaB reverse	TCCTTCCTGTTGAACACCCTCT
17	flaB probe	AAACTGCTCAGGCTGCACCGGTTC
18	lacZBb forward	TTCTCTTGGAGGATTTGCTAAAT
19	lacZBb reverse	ATCCCAAACAAATCCACCTT
20	lacZBb probe	TGGCAAGCATTCAGACAATATCCAAG
21	ospC forward	ACGGATTCTAATGCGGTTTTACTT
22	ospC reverse	CAATAGCTTTAGCAGCAATTTCATCT
23	ospC probe	CTGTGAAAGAGGTTGAAGCGTTGCTGTCAT

^aPrimer no. 15 to 23 are TaqMan primers/probes.

^bSequence portions in bold denote recognition sites for restriction enzymes (which are indicated in parentheses after the oligonucleotide name).