TABLE 1.
In-house-prepared paramagnetic-bead-coating-antigen combinations evaluated
| Paramagnetic beads | Coating antigena |
||||
|---|---|---|---|---|---|
| Polyclonal antibody S624 | aMp3 peptide | aMptD peptide | Biotinylated aMp3 peptide | Biotinylated aMptD peptide | |
| Dynabeads, M280 sheep anti-rabbit IgGb | + | − | − | − | − |
| Magnabind carboxyl derivatized beadsc | − | + | + | − | − |
| Amine-coated magnetic hollow glass microspheresd | + | + | + | − | − |
| Dynabeads, MyOne Carboxylic acidb | + | + | + | − | − |
| Dynabeads, MyOne Tosylactivatedb | + | + | + | + | + |
| Dynabeads, MyOne Streptavidin-T1b | − | − | − | + | + |
| Dynabeads, M280 Streptavidinb | − | − | − | + | + |
a
+, tested; −, not tested.
b
From Invitrogen, Life Technologies Corporation.
c
From Pierce Protein Research Products, Thermo Scientific.
d
From Microsphere Technology Limited, Adare, County Limerick, Republic of Ireland.