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. 2010 Sep 10;76(21):7029–7035. doi: 10.1128/AEM.00464-10

FIG. 2.

FIG. 2.

Growth- and dose-dependent induction of GFP by benzoate. Error bars represent 1 standard deviation from the mean (n = 4). (A) Growth phase dependence of GFP fluorescence. E. coli JM109/pCmGFPbenR sensor cells were grown to the log or stationary phase, and then benzoate was added to the culture medium (final concentration of 0.5 mM). Cell density (left panel) and fluorescence intensity (right panel) were monitored after induction. Open circle, log-phase cells (no benzoate induction); filled circle, log-phase cells induced with 0.5 mM benzoate; closed triangle, stationary-phase cells induced with 0.5 mM benzoate. (B) GFP fluorescence in sensor cells (E. coli JM109/pCmGFPbenR) induced with different concentrations of benzoate. The inset panel shows a close-up of the graph in the 0 to 100 μM benzoate region.