TABLE 3.
Relative activities of the 11 clone types on different amide substrates
| Substrate | Relative activity of the indicated clone carrying a recombinant plasmid (nmol/min/mg of protein)a |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pHSG398b | 1B9 | 3B3 | 3B10 | 3D8 | 4E7 | 5C8 | 6H10 | 8D | 9D11 | 10A5 | 10H1 | |
| Benzamide | <12 | 160.9 ± 3.2 | 233.0 ± 7.7 | 263.5 ± 14.1 | 36.4 ± 3.2 | 149.3 ± 5.6 | 108.6 ± 2.3 | 277.8 ± 6.0 | 176.0 ± 9.3 | 72.2 ± 7.2 | 266.6 ± 10.2 | 115.7 ± 10.3 |
| Acetamide | <12 | <12 | <12 | <12 | <12 | 22.3 ± 0.5 | 13.3 ± 0.4 | 13.0 ± 0.4 | 13.7 ± 1.7 | <12 | <12 | 13.6 ± 0.6 |
| Acrylamide | <12 | 16.4 ± 0.3 | <12 | <12 | <12 | 15.9 ± 0.7 | <12 | <12 | 21.2 ± 1.2 | <12 | <12 | <12 |
| Indole actamide | <12 | 174.0 ± 3.7 | 27.0 ± 0.9 | 15.2 ± 0.2 | 73.5 ± 4.4 | 217.1 ± 2.4 | 24.0 ± 0.3 | 30.9 ± 0.7 | 181.8 ± 5.6 | 60.3 ± 5.3 | 20.7 ± 0.1 | 84.2 ± 0.2 |
| Nicotine amide | 17.5 ± 2.4 | 153.6 ± 4.5 | 83.6 ± 1.3 | 56.8 ± 0.4 | 31.8 ± 1.5 | 68.8 ± 1.0 | 52.8 ± 1.1 | 265.1 ± 2.2 | 175.6 ± 3.7 | 76.9 ± 3.8 | 287.8 ± 3.5 | 77.9 ± 1.0 |
| Propionamide | <12 | 149.4 ± 5.1 | 35.9 ± 0.9 | <12 | 17.5 ± 4.2 | 141.2 ± 5.1 | <12 | 22.7 ± 0.9 | 177.2 ± 12.1 | 55.8 ± 5.1 | 15.9 ± 0.4 | <12 |
| Glycine amide | <12 | 61.9 ± 4.8 | 137.3 ± 4.8 | 127.0 ± 6.2 | 46.5 ± 6.2 | 122.5 ± 5.4 | 136.4 ± 3.3 | 128.3 ± 3.7 | 52.9 ± 13.0 | 55.5 ± 8.5 | 127.3 ± 9.0 | 123.7 ± 5.7 |
| l-Alanine amide | <12 | 92.2 ± 1.8 | 118.1 ± 1.8 | 108.4 ± 3.4 | 37.6 ± 1.5 | 107.6 ± 3.4 | 124.3 ± 7.2 | 117.6 ± 2.6 | 61.9 ± 3.4 | 42.8 ± 10.7 | 114.2 ± 5.5 | 112.0 ± 4.2 |
| d-Alanine amide | <12 | 124.8 ± 15.1 | 121.7 ± 8.8 | 108.5 ± 0.8 | 36.2 ± 3.2 | 105.0 ± 2.3 | 118.3 ± 2.8 | 114.1 ± 2.4 | 92.4 ± 5.9 | 57.4 ± 2.3 | 116.4 ± 9.9 | 108.5 ± 3.8 |
| l-Leucine amide | <12 | 120.8 ± 5.4 | <12 | <12 | 27.5 ± 2.5 | <12 | <12 | <12 | 100.1 ± 2.7 | 51.6 ± 5.4 | <12 | <12 |
| d-Leucine amide | 20.2 ± 2.2 | 116.3 ± 6.4 | <12 | <12 | 104.3 ± 4.7 | <12 | <12 | <12 | <12 | 103.7 ± 2.4 | <12 | <12 |
| l-Proline amide | <12 | 103.9 ± 11.3 | <12 | <12 | <12 | <12 | <12 | <12 | 39.6 ± 2.2 | 60.7 ± 3.3 | <12 | <12 |
| d-Proline amide | <12 | 101.6 ± 8.4 | <12 | <12 | <12 | <12 | <12 | <12 | 54.8 ± 3.8 | 67.4 ± 1.7 | <12 | <12 |
| l-Phenylalanine amide | 18.9 ± 1.4 | 112.9 ± 10.9 | <12 | <12 | 101.7 ± 4.9 | 180.6 ± 4.8 | <12 | <12 | 99.3 ± 3.2 | 107.3 ± 3.5 | <12 | <12 |
| d-Phenylalanine amide | 30.9 ± 1.9 | 111.5 ± 10.7 | <12 | <12 | 98.3 ± 3.9 | 206.2 ± 7.2 | <12 | 15.9 ± 0.5 | 57.4 ± 2.6 | 102.0 ± 4.1 | <12 | <12 |
a
Reactions were done under the standard conditions described in Materials and Methods. All measurements were done in triplicate.
b
E. coli JM109 was transformed by pHSG398.