FIG. 3.

Evolution of the number of sublethally damaged cells (closed circles, continuous lines) during an HHP treatment (450 MPa, 60 min) of stationary-phase cultures of S. aureus CECT 4459 (A) and CECT 4465 (B). Also included are the survival curves (open symbols, discontinuous lines) when HHP-treated cultures were plated on nonselective medium (□) and selective medium (▵). The initial temperature was 25°C, the treatment medium was citrate-phosphate buffer, pH 7.0, the initial cell concentration was 2 × 10⁸ to 6 × 10⁸ cells/ml, the recovery medium was TSA-YE (nonselective medium) or TSA-YE-NaCl (selective medium), and the recovery atmosphere condition was aerobiosis.