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. 2010 Sep 17;76(21):7363–7366. doi: 10.1128/AEM.01326-10

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FIG. 2. — Heterologous expression and purification of recombinant Xyn10A. (A) Zymogram assay showing xylanase activity following electrophoresis of crude cell extracts. Lanes: ST, molecular weight standards; 1 and 3, crude cell extracts from DH5α(pK19) (vector control); 2 and 4, crude cell extracts from DH5α(pK19-xyn10A). Samples in lanes 3 and 4 were heated at 65°C for 15 min prior to loading. (B) SDS-PAGE (10% gel) showing purification of Xyn10A. Lanes: A, molecular weight standards; B, crude cell extract from DH5α(pK19-xyn10A); C, heat-treated extract from DH5α(pK19-xyn10A); D, concentrated fractions from the hydroxyapatite column.