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Time course of Xyn10A product formation with birchwood (A) and oat spelt (B) xylans, using TLC. Xylan substrates (2% in 10 mM phosphate buffer, pH 6) were incubated with purified Xyn10A at 90°C. Lanes 1 and 8, standards: xylotetraose (X4) and xylotriose (X3) (Megazyme, Wicklow, Ireland) and xylose (X1). Lane 2, unreacted xylan; lanes 3 to 7, xylan with increased incubation times in the presence of purified Xyn10A (10, 20, 40, 60, and 240 min, respectively).
