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Typical purification of Xyn10A from recombinant E. coli
| Purification step | Amt of protein (mg) | Activitya |
Yield (%) | Purification (fold) | |
|---|---|---|---|---|---|
| Units | Units/mg protein | ||||
| Crude cell extract | 3,840 | 14,800 | 3.85 | 100 | |
| Heat-treated extract | 838 | 13,100 | 15.6 | 88 | 4.1 |
| Hydroxyapatite | 4.63 | 1,740 | 376 | 12 | 97.7 |
Units, μmol xylose-reducing equivalents liberated from oat spelt xylan per min; activities measured at 90°C and pH 6.0.
