TABLE 2.
MS analysis and antimicrobial activities of some selected fractions from the last step of purification
| Fraction | MS peak(s) (Da) | Antimicrobial activity (BU/mg)b |
|---|---|---|
| Nr 23 | 3,877 | 0 |
| Nr 24 | 5,065, 5,306a | 22,200 |
| Nr 25 | 3,678, 5,063, 5,306 | 5,000 |
| Nr 26 | 3,678, 5,063 | 0 |
a
Major peak; see Fig. 1.
b
One BU (bactericidal unit) is the minimum amount of the antimicrobial peptide that inhibits growth of the indicator by 50%. The protein concentration is based on a value of 1 mg/ml at an optical density at 280 nm of 1.88; the protein concentrations in fractions 24 and 25 were 18 μg/ml and 20 μg/ml, respectively.