TABLE 2.
Relevant features of the plasmids used in this work
| Plasmid | Characteristics | Reference or source |
|---|---|---|
| pBgD12S | Apr, as-48A cloned into pSL1180 (5.4 kb) | 48 |
| pBgC | Apr, BglII-BglII (fragment C) from as-48 cluster cloned into pSL1180 (9.2 kb) | 8 |
| pBgD12SMut | Apr, pBgD12S with the His-1Ile, Met1Ala, or Trp70Ala substitution in as-48A (5.4 kb) | This work |
| pAM401 | Cmr, Tcr, cloning expression bifunctional E. coli-E. faecalis vector (10.4 kb) | 61 |
| pAM401-76 | Cmr, D and B fragments (as-48A, as-48G, as-48H genes) from pMB2 cloned into pAM401 (18.9 kb) | 8 |
| pAM401-76Mut | Cmr, pAM401-76 with the His-1Ile, Met1Ala, or Trp70Ala substitution in as-48A (18.9 kb) | This work |
| pAM401-81 | Cmr, as-48 cluster cloned in pAM401 (25 kb) | 8 |
| pAM401-81Mut | Cmr, pAM401-81 with the His-1Ile, Met1Ala, or Trp70Ala substitution in as-48A (25 kb) | This work |