TABLE 4.
Diameters of inhibition halos produced by the JH2-2(pAM401-81Mut) transformants against different Gram-positive strains, determined using the spot assay techniquea
| Gram-positive strain | Halo diam (mm) |
|||
|---|---|---|---|---|
| AS-48 | His-1Ile | Met1Ala | Trp70Ala | |
| Arthrobacter sp. | 31 | — | 17 | 21 |
| Bacillus cereus LWL1 | 16.5 | — | 5 | 13.5 |
| Bacillus megaterium CECT 44 | 25 | — | 11 | 19 |
| Enterococcus faecalis JH2-2 | 21 | — | 13 | 18 |
| Enterococcus faecalis S-47 | 21 | — | 12 | 17 |
| Listeria monocytogenes CECT 4032 | 27 | — | 14 | 21.5 |
| Listeria innocua CECT 4030 | 23 | — | 12 | 17 |
| Staphylococcus aureus CECT 976 | 16 | — | — | 12 |
a
The activity of JH2-2(pAM401-81) was determined as a control. —, no activity.