FIG. 6.

Galactose consumption and time courses for α-Gal1P, α-G1P, and FBP pools in resting cells of L. lactis strains engineered to improve galactose utilization. [1-¹³C]galactose (20 mM) was supplied at time 0 min to nongrowing suspensions of strains NZ9000(pGalPMKT) (A), NZ9000(pGalPMKTpgmA) (B), control strain NZ9000(pNZ8048) (C), and NZ9000(pGalPpgmA) (D); and its metabolism was monitored by in vivo ¹³C NMR under anaerobic conditions at pH 6.5 and 30°C. Maximal galactose consumption rates (μmol min⁻¹ mg protein⁻¹) are boxed in the upper-left corners. Symbols: closed diamond, galactose; closed triangle, fructose 1,6-bisphosphate; open circle, α-glucose 1-phosphate; gray diamond, α-galactose 1-phosphate.