TABLE 2.
Primers used in this study
| Primer | Sequence (5′ to 3′) | Enzyme |
|---|---|---|
| GalA1-fw | CGGTCTCCCATGAAAGAGGGAAAAATGAAACAACG | Eco31I |
| GalA-rev | CTAGTCTAGATTATTTCAAACGTTCTTC | XbaI |
| GalT-rev | CTAGTCTAGATTATTGATTCACAAAATC | XbaI |
| PgmA-fw1 | CATGCCATGGTAGTTGTGATACAATGTAAGCG | NcoI |
| PgmA-fw2 | GCTCTAGATAGTTGTGATACAATGTAAGCG | XbaI |
| PgmA-rev | GCTCTAGATTGGTGTAGCAGCGAAAG | XbaI |
| GalA-KO1 | GCTCTAGACTTTCGGGAGAAACCGTGG | XbaI |
| GalA-KO2 | CGGGATCCCCCTCTTTCATGGGAATCC | BamHI |
| GalA-KO3 | CGGGATCCCCTTTGTAGTCCCAGCGG | BamHI |
| GalA-KO4 | CGGAATTCGAATGCTATCTTCTCCACC | EcoRI |
| GalAMK-KO5 | CGGGATCCGATGATTACGAAGTCACTGG | BamHI |
| GalAMK-KO6 | CGGAATTCAATCGCCAGAAGTTGGTCC | EcoRI |
| LacFE-fw | CCTGATCATGAACAGAGAAGAGATGAC | RcaI |
| LacFE-rev | TGCTCTAGATTAATCAAACTGTTGTTG | XbaI |
| LacAD-fw | AAACTGCAGATGGCTATTGTTGTTGGTGC | PstI |
| LacAD-rev | GGACTAGTCTATACTTTATCAGTCCATGGAC | SpeI |
| LacAE-fw | ACATGCATGCATGGCTATTGTTGTTGGTGC | SphI |
| LacAE-rev | GGACTAGTTTAATCAAACTGTTGTTGAACAAATG | SpeI |
| LacG-fw | CGAGCTCAATATATCAAATTGACACGTGACGG | SacI |
| LacG-rev | CGAGCTCTTACTCTATCACTTGAGTTTCTGC | SacI |