MboI resistance among swine-derived C. coli. Genomic DNA was extracted with a Qiagen DNeasy kit and digested with Sau3AI and MboI. Lanes (after lane 1, the sets of three lanes for the isolates contain DNA that is undigested, digested with Sau3AI, and digested with MboI, respectively): 1, molecular size markers (Roche, Indianapolis, IN); 2 to 4, DNA of the turkey-derived isolate 6017; 5 to 7, DNA of the turkey-derived isolate 6067; 8 to 10, DNA of the swine-derived isolate 6458; 11 to 13, DNA of the swine-derived isolate 6461; 14 to 16, DNA of the swine-derived isolate 6550; 17 to 19, DNA of the swine-derived isolate 7995.