TABLE 3.
Classification of eukaryotic clones retrieved from the biofilm in the BBT flask after ≥20 days of incubation at 37°C with samples from drinking water supplies, surface water, and cooling towers inoculated with L. pneumophila
| Kingdom or subkingdom | Drinking water supplies |
Surface water |
Cooling towers |
All analyzed samples |
||||
|---|---|---|---|---|---|---|---|---|
| No.a of OTUs (%) | % of clone libraries | No. of OTUs (%) | % of clone libraries | No. of OTUs (%) | % of clone libraries | No. of OTUs (%) | % of clone libraries | |
| Free-living protozoa | 35 (40.4) | 66.4 | 7 (25.0) | 34.8 | 30 (56.6) | 57.4 | 68 (41.2) | 57.7 |
| Amoebozoa | 8 (22.2) | 58.2 | 4 (57.1) | 57.4 | 21 (70) | 66.7 | 29 (42.6) | 59.7 |
| Cercozoa | 8 (22.2) | 8.4 | 3 (42.9) | 42.6 | 5 (16.7) | 15.2 | 17 (25) | 14.2 |
| Choanozoa | 11 (30.6) | 25.9 | 0 (0.0) | 0 | 3 (10) | 16.7 | 14 (20.6) | 20.7 |
| Euglenozoa | 2 (5.6) | 5.0 | 0 (0.0) | 0 | 0 (0.0) | 0 | 2 (2.9) | 3.4 |
| Myzozoa | 5 (13.9) | 2.1 | 0 (0.0) | 0 | 0 (0.0) | 0 | 5 (7.4) | 1.4 |
| Stramenopiles | 1 (2.8) | 0.4 | 0 (0.0) | 0 | 1 (3.3) | 1.5 | 2 (2.9) | 0.6 |
| Fungi | 25 (28.1) | 15.8 | 18 (64.3) | 60.7 | 17 (32.1) | 31.3 | 60 (36.4) | 28.7 |
| Metazoa | 14 (15.7) | 10.8 | 0 (0) | 0 | 2 (3.8) | 4.3 | 14 (8.5) | 7.2 |
| Protophyta and plants | 3 (3.4) | 1.1 | 3 (10.7) | 4.4 | 2 (3.8) | 3.5 | 8 (4.8) | 2.3 |
| Organisms with <75% similarity | 12 (13.5) | 5.8 | 0 (0) | 0 | 2 (3.8) | 3.5 | 15 (9.1) | 4.1 |
| Total | 89 (100) | 100 | 28 (100) | 100 | 53 (100) | 100.0 | 165 (100) | 100 |
a
OTUs obtained from more than one sample type are included only once.