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. 2010 Sep 17;76(21):7109–7115. doi: 10.1128/AEM.01702-10

FIG. 4.

FIG. 4.

Quantitation of the peroxisome numbers in P. chrysogenum strains that vary in the number of penicillin biosynthetic gene clusters. (A to C) Visualization of hyphae and peroxisomes through DS-RED fluorescence, as recorded by CLSM of strain DS47274 containing a single penicillin gene cluster, as shown in the following images: bright-field image (A), DS-RED fluorescence image (B), and merged image (C). (D) The numbers of peroxisomes per 1,000 μm3 in the DS47274, DS47276, and DS17690 strains were counted and plotted against the penicillin cluster copy numbers. The hyphae of the different cells had identical diameters and dimensions.