FIG. 6.

Comparison of two single penicillin biosynthetic gene cluster strains, P. chrysogenum Wisconsin54-1255 (white bars) and DS47274 (black bars). (A) Quantification of the penicillin cluster copy number; (B) relative expression levels of pcbAB, penDE, pcbC, and phl; (C) relative protein levels of ACVS, AT, IPNS, and PCL; (D) extracellular levels of penicillin after 5 and 7 days. Samples were taken after 5 days of fermentation, unless indicated otherwise. The transcript and protein levels of DS47274 were set at 12.5%, as described in the legends to Fig. 2 and 3.