Fig. 7.

Temporal requirement of Jag1 signaling for morphogenesis of bile ducts. (A) Notch signaling blockade in wild-type co-cultures of PVM and BECs (at E16.5 and P2) with DAPT prevents spheroid formation (arrows) at E16.5 but not at P2. (B) Western blot demonstrates Notch2 activation within BECs after binding to Jag1 peptide (Fc, control). Black arrow indicates intact Notch2; black arrowhead indicates cleaved Notch2. When cultured with J1^SMKO PVM, E16.5 BECs previously activated with soluble Jag1 peptide regained the ability to form spheroids (arrows), whereas BECs treated with Fc control peptide did not. Scale bars: 100 μm in A,B.