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. 2010 Nov;9(11):1728–1733. doi: 10.1128/EC.00141-10

Fig. 5.

Fig. 5.

RasC and RasD activation in response to cAMP. Extracts from rasD-, rasG-/[rasG]:rasD, and rasC-/rasG-/[rasG]:rasD cells that had been pulsed with cAMP and then subjected to a single cAMP stimulus for the indicated times (in seconds) were bound to GST-Byr2-RBD as described in Materials and Methods. The bound material was analyzed by Western blotting, using antibodies specific for RasC (A) or RasD (B). The “Total” lanes represent the level of Ras protein in 8.3 μg of lysate protein used for the pull-down assay.