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Four factors required for growth are restricted to the filament tip. The indicated polarity factors are enriched at the growing end of the filaments (strains JA1523, JA1543, JA1577, and JA1545). Single cells are shown at division and before and after new-end take-off (pre-NETO and post-NETO, respectively). Fluorescent images are maximum-intensity stacks; light transmission images are single planes. Bars, 5 μm.
