Table 1.
S. pombe strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source | Reference |
|---|---|---|---|
| Strains | |||
| 972 | h− | Our collection | |
| 556 | h+ade6-216 ura4-D18 leu1-32 | Our collection | |
| UFMG-96-A1153a | Wild type | C. Rosa | 8 |
| FY14674 | h90 ade6-216 leu1-32 lys1-131 ura4-D18 crn1::crn1-GFP-HA-Kanr | Y. Hiraoka/YGRCb | |
| NM01 | cdc25-22 bud6-3GFP:kanMX | F. Chang | 18 |
| NM15 | cdc25-22 leu1−::GFP-bgs4:leu1+ | F. Chang | 18 |
| NM39 | cdc25-22 tea1-3GFP:kanMX | F. Chang | 18 |
| NM145 | cdc25-22 CRIB-GFP:ura4+ | F. Chang | 18 |
| YSM120 | h+tea4-GFP-kanMX6 ade6−leu1-32 ura4-D18 | F. Chang | 14 |
| YSM1342 | h−nif1-GFP-kanMX | S. Martin | 13 |
| PT.907 | cdc25-22 GFP-mod5:kanRleu1-32 h+ | Phong Tran | 33 |
| JM414 | pom1-GFP::kanMX6 h− | J. Moseley | 2 |
| JM430 | Mid1-mEGFP::kanMX6 ura4-D18 leu1-32 ade6-M21X h+ | J. Moseley | 26 |
| CA5818 | h−leu1-32 ura4-D18 for3:3GFP(kanMX6) | K. Shiozaki | 31 |
| CA4699 | h−leu1-32 ura4-D18 rga4:GFP(kanMX6) | K. Shiozaki | 31 |
| KGY2628 | h−sid4-GFP-kanR ade6-M210 ura4-D18 leu1-32 | K. Gould | 34 |
| DL04A03 | h+ade6-m216 ura4-D18 leu1-32 tea1::KanMX4 | Bioneer | |
| V2-22-B02 | h+ade6−ura4-D18 leu1-32 lys1::KanMX4 | Bioneer | |
| JA1375 | Strain 556 carrying p02/G12 (leu1-32::htb1-YFP-leu1+) | This study | |
| JA1374 | Strain 556 carrying p18/E01 (leu1-32::spo20-YFP-leu1+) | This study | |
| JA1530 | ura4-D18 lys1::KanMX4 (selected from crossing strains A1153 and V2-22-B02) | This study | |
| JA1568 | leu1-32 lys1::KanMX4 (selected from crossing strains A1153 and V2-22-B02) | This study | |
| JA1551 | leu1-32::htb1-YFP-leu1+ (selected from crossing strains JA1568 and JA1375) | This study | |
| JA1577 | leu1-32::spo20-YFP-leu1+ (selected from crossing strains JA1568 and JA1374) | This study | |
| JA1523 | crn1::crn1-GFP-HA-Kanr (selected from crossing strains A1153 and FY14674) | This study | |
| JA1543 | CRIB-GFP:ura4+ (selected from crossing strains JA1530 and NM145) | This study | |
| JA1545 | leu1−::GFP-bgs4:leu1+ (selected from crossing strains JA1551 and NM15) | This study | |
| JA1476 | bud6-3GFP:kanMX (selected from crossing strains A1153 and NM01) | This study | |
| JA1529 | for3:3GFP(kanMX6) (selected from crossing strains A1153 and CA5818) | This study | |
| JA1489 | tea1-3GFP:kanMX (selected from crossing strains A1153 and NM39) | This study | |
| JA1542 | tea4-GFP-kanMX6 (selected from crossing strains A1153 and YSM120) | This study | |
| JA1578 | GFP-mod5:kanR (selected from crossing strains A1153 and PT.907) | This study | |
| JA1500 | pom1-GFP::kanMX6 (selected from crossing strains A1153 and JM414) | This study | |
| JA1501 | Mid1-mEGFP::kanMX6 (selected from crossing strains A1153 and JM430) | This study | |
| JA1527 | rga4:GFP(kanMX6) (selected from crossing strains A1153 and CA4699) | This study | |
| JA1556 | nif1-GFP-kanMX (selected from crossing strains A1153 and YSM1342) | This study | |
| JA1567 | tea1::KanMX4 (selected from crossing strains A1153 and DL04A03) | This study | |
| JA1576 | sid4-GFP-kanR (selected from crossing strains A1153 and KGY2628) | This study | |
| JA1240 | Strain 556 carrying pREP41GFP-c att c CpY1 | N. Bone | |
| JA1582 | Strain 556 carrying pREP41GFP-c att c CpY1 (selected from crossing strains JA1551 and JA1240) | This study | |
| Plasmids | |||
| p02/G12 | pDUAL nmt42-htb1-HA-YFP-ura4+/leu1+ | M. Yoshida/Riken BRCc | 17 |
| p18/E01 | pDUAL nmt42-spo20-HA-YFP-ura4+/leu1+ | M. Yoshida/Riken BRC | 17 |
a
Strain UFMG-96-A1153 is referred to as A1153 hereafter.
b
Y. Hiraoka/YGRC, Y. Hiraoka of the Yeast Genetic Resource Centre (YGRC).
c
M. Yoshida/Riken BRC, M. Yoshida of the Riken BioResource Center (Riken BRC).