Fig. 5.

Crh2-HA secretion is dependent on the unfolded protein response and independent of ERAD components. (A) The indicated strains expressing CRH2-HA were analyzed by colony immunoblotting as described in the legend of Fig. 2. (B) CRH2-HA was expressed in strains with mutations in the UPR (hac1Δ), GPI trafficking (emp24Δ and erv25Δ), and ERAD (htm1Δ, doa10Δ, and hrd1Δ) and analyzed by colony immunoblotting. (C) The indicated strains were spotted onto plates of synthetic dextrose medium lacking uracil as a 10-fold dilution series and incubated for 2 days at 30°C. (D) The localization of Crh2-GFP in wt and ire1Δ cells was analyzed as described in the legend of Fig. 1. The arrows indicate the expected budneck/septum localization in both strains (24).