Fig. 7.

Genetic interactions of YPS1 and YPS2 with the unfolded protein response. (A) The indicated strains were streaked onto plates containing YPD and YPD plus 2-deoxyglucose (DG) and incubated at 30°C for 2 days. (B) WT, ire1Δ, GAL-CPY*, and ire1Δ GAL-CPY* strains harboring a plasmid with the promoter of YPS1 fused to lacZ were grown to the logarithmic phase and treated with tunicamycin to induce ER stress or galactose to induce the expression of the misfolded protein CPY*. After 5 h of exposure to the stress condition, the cells were harvested, and the β-galactosidase activity of cellular extracts was assayed as described in the legend of Fig. 3.