Time course of pheromone-induced cell death in different C. albicans mutants. (A to D) Opaque MTLa strains lacking the FIG1, FUS1, CNB1, or MKC1 gene were treated with 10 μM α pheromone, and the percentage of dead cells was determined by staining with propidium iodide and analysis by flow cytometry. (E) Evaluation of PID in the presence or absence of 2.5 μM FK506. Wild-type, Δfus1, and Δfig1 cells were treated with 10 μΜ pheromone in the presence or absence of FK506 for 5 h and then stained with propidium iodide and analyzed by flow cytometry.