Fig. 6.

Secretion of ACW-1 is elevated in the och-1 mutant. Western blot analysis of protein using antibody directed against ACW-1. Lane 1, wild-type cytosolic fraction (30 μg of cytosolic protein); lane 2, och-1 cytosolic fraction (30 μg of cytosolic protein); lane 3, wild-type secreted protein fraction (normalized to the amount of secreted protein from 30 μg of cytosolic protein); lane 4, och-1 secreted protein fraction (normalized to the amount of secreted protein from 30 μg of cytosolic protein); lane 5, wild-type secreted protein fraction (normalized to the amount of secreted protein from 500 μg of cytosolic protein); lane 6, och-1 secreted protein fraction (normalized to the amount of secreted protein from 500 μg of cytosolic protein).