Fig. 6.

Disruption of host microtubules has no apparent affect on parasite contact, intimate attachment, or gliding motility. (A) GFP⁺ parasites in high-K⁺ buffer were added to mock-treated (▪) or nocodazole-treated (□) HFFs and switched to invasion medium for the indicated times. The medium was then removed, and the cells were fixed without washing. The numbers of cell-associated GFP⁺ parasites and host nuclei were counted. Means and standard deviations from three independent assays counting 10 random fields for each sample are shown. (B) GFP⁺ parasites in high-K⁺ buffer were added to mock-treated (▪) or nocodazole-treated (□) HFFs. The medium was removed, after which the cells were washed three times with ice-cold PBS and then fixed. The numbers of cell-associated GFP⁺ parasites and host nuclei were counted. The means and standard deviations from three independent experiments counting 10 random fields in each sample are shown. (C) GFP⁺ parasites were added to formaldehyde-fixed and washed mock- or nocodazole-treated cells for 60 min at 37°C. The cells were fixed and stained with anti-SAG1. Representative images from three independent experiments are shown.