Table 1.

E. histolytica glycoproteins with occupied N-glycan sites as shown by PNGaseF treatment and mass spectrometry

Accession no.	Protein name or description	Mascot score	Sequence coverage (%)	Glycosylation site(s)a
EHI_012270	Gal/GalNAc lectin heavy subunit	2,478	43	ANLTER
EHI_133900	Gal/GalNAc lectin heavy subunit	2,007	45	QYNTSCEPK, VSNCTEDLVR
EHI_006980	Gal/GalNAc lectin intermediate subunit	1,011	36	NVSNDCECNDKHIPTSIDK, DGFYQIENATDGVYCSPCPAK
EHI_065330	Gal/GalNAc lectin intermediate subunit	959	43	SPYSNCTTCIESNYYPK
EHI_077530	Unique GPI-anchored glycoprotein	201	37	NETSVAVSEENFK
EHI_138750	Receptor-kinase	143	13	YDVPYNESLDK, SCQTCNSTIDPK
EHI_093710	Secreted glycoprotein similar to Igl1	141	34	VNGSCIQTIDACDTYVNPLFEK, YNFYLPTNGTCER
EHI_161040	Unique secreted protein with PKEDQ repeats	384	42	ENECNETEEYK
EHI_008120	Unique type 1 membrane glycoprotein	201	18	SFLLNITK
EHI_109920	Unique basic secreted glycoprotein	198	26	NVTDYDVHSVR
EHI_054530	Serine carboxypeptidase	151	12	YFINDTIYSK
EHI_151440	Cysteine proteinase	138	27	DYPYTATNGTCQYDADK
EHI_093970	Cysteine proteinase	133	16	FNVTSITTK
EHI_188160	Unique ER glycoprotein	107	23	KPIAYFTNSTLGLTK
EHI_022130	Unique secreted glycoprotein	100	29	IPTENR(T), VNASIIESYKK
EHI_133530	Unique secreted glycoprotein	78	31	VNASIIESLK, ALSDAVSNVLPNTTDK
EHI_194850	Unique secreted glycoprotein	65	8	INVIDAFEGDTNLTIPK
EHI_178470	Unique type 1 transmembrane glycoprotein	62	9	TNYSFPSPPSVSEYSDFK
EHI_096530	Asp-rich type 1 membrane glycoprotein	62	8	YISSVNCSATENYETK
EHI_024530	Unique type 1 membrane glycoprotein	52	23	EIVNVTGQIGTLITR
EHI_098000	Unique type 1 membrane glycoprotein	51	17	VSSGNVSYR
EHI_180770	Unique type 1 membrane glycoprotein	50	15	YIQGDDNVINR
EHI_048140	Unique type 2 membrane glycoprotein	50	28	VSNTTDSGNATSTK
EHI_185260	Unique type 1 membrane glycoprotein	50	5	FGYASLFADPTNCSVR
EHI_107300	Unique GPI-anchored glycoprotein	50	23	IYCDPVEGPIIFNFNNSK

^aBoldface indicates where the predicted Asn was converted to Asp by PNGaseF treatment.