Fig. 2.

The vcx1 mutant displays calcineurin-dependent Ca²⁺ sensitivity. Ten-fold serial dilutions of WT H99, vcx1 mutant (vcx1Δ), and vcx1::VCX1-complemented (vcx1Δ::VCX1) cells were plated in YPD agar containing 100 μg/ml of CsA (A), 100 or 200 mM CaCl₂ (B), 50 mM CaCl₂ amended with 100 μg/ml of CsA (C), 4 mM MnCl₂ or 50 μM CdCl₂ (D), or 4 mM MnCl₂ or 50 μM CdCl₂ amended with 100 μg/ml of CsA (E). The plates were incubated for 2 days at 30°C, 35°C, or 37°C, as indicated. As control, cells were grown in YPD agar only.