Table 2. The position of G nucleotide in the targets when the reduction of fluorescence after hybridization was observed.

		F0	F	(F0 – F)/F0
5′-ACAAAAAAAAAATATATATA-3′a 3′-TTTTTTTTTTTGTTTTTTTTTTATATATAT-5′b		573.3	564.5	0.02
5′-CAAAAAAAAAAATATATATA-3′a 3′-TTTTTTTTTTGTTTTTTTTTTTATATATAT-5′b		521.3	146.2	0.72
5′-AAAAAAAAAAAATATATATA-3′a 3′-TTTTTTTTTGTTTTTTTTTTTTATATATAT-5′b		489.6	253.4	0.48
5′-AAAAAAAAAAAATATATATA-3′a 3′-TTTTTTTTGTTTTTTTTTTTTTATATATAT-5′b		479.7	406.2	0.15

F₀, fluorescence intensity (arbitrary units) without the target DNA; F, fluorescence intensity after the addition of the target DNA.

^aProbe sequence.

^bTarget sequence.