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. 2010 Nov;9(11):1702–1710. doi: 10.1128/EC.00106-10

Fig. 2.

Fig. 2.

Localization of LDK-V5 to lipid droplets. Images of control parasites (uninduced, Tet−) and or those induced for expression of LDK-V5 (Tet+) were collected using the same parameters and identically processed. Transfectants expressing LDK-V5 were stained with Nile Red to identify lipid droplets and costained with anti-V5 antibodies (green). Nile Red was collected on the Texas Red filter, while LDK-V5 was visualized in the green channel. Cells were costained with DAPI (blue). All bars, 5 μm. (A) Localization of LDK-V5 to lipid droplets in PF. Left, induced for LDK-V5 expression (+Tet); right, uninduced (−Tet). (B) Localization of LDK-V5 to lipid droplets in BF. (C) Deletion of the hydrophobic region of LDK leads to cytosolic localization in PF. Due to the lower maximum signal intensity, the scaling for brightness is twice that of the images showing wild-type protein. (D) Threefold enlargement of lipid droplet staining of panel cell immediately above. (E) Tet-induced PF cell showing LDK-V5 localization to the ER and lipid droplets (green). Anti-BiP was used as a marker of the ER (red).