Fig. 4.

RNAi targeting LDK in PF and BF does not abrogate parasite proliferation. (A) Northern analysis of PF transfectants bearing an RNAi cassette targeting LDK. The abundance of full-length 3.32-kb mRNA seen in the uninduced condition (Tet−) is dramatically reduced upon induction of RNAi (Tet+). The small species in the Tet+ lane corresponds to the double-stranded RNA produced upon induction. At right is the control hybridization with α-tubulin. Migration of molecular mass markers is shown (in kb). (B) Growth curve for PF, induced (Tet+) or uninduced (Tet−) for RNAi. Standard deviations of cell counts are obscured by markers. (C) Growth curve for BF (Tet+) or uninduced (Tet−) for RNAi. Standard deviations of cell counts are obscured by markers.