Fig. 7.
Growth in delipidated serum increases LDK autophosphorylation activity. (A) PF induced for expression of LDK-V5 were grown for 4 h in medium containing delipidated or normal serum. The tagged LDK was immunoprecipitated and subjected to a kinase assay as in Fig. 1, measuring autophosphorylation (32P) and the amount of LDK-V5 by Western blotting (anti-V5) on the same lanes. U, untreated; D, grown in delipidated serum. (B) Graph of the relative activity of LDK from cells grown in delipidated serum versus cells from a parallel culture grown in normal serum. Shown are the data from two independent isolates (Iso 1 and Iso 2), and two independent experiments for isolate 2 (Exp1 and Exp2).