Fig. 1.

C. albicans Aps3p and Vps21p facilitate distinct vacuolar trafficking pathways. (A) Each strain was pulse-labeled with the endocytic marker FM4-64 and chased for 2 h in fresh medium at 30°C. Cells were then observed by deconvolution fluorescence microscopy to see FM4-64 (red) and Nomarski optics; merged images are shown. Arrowheads indicate internal vesicles within the vacuole lumens of aps3Δ/Δ and aps3Δ/Δvps21Δ/Δ cells. Scale bar, 5 μM. (B) ALP activity was assayed from cell extracts of each strain and expressed as a percentage of control strain (YJB6284) activity. Results presented are the means and standard deviations of results of three independent experiments. *, P < 0.05; **, P < 0.0001. WT, wild type.