Fig. 3.

Loss of both APS3 and VPS21 leads to synthetic stress phenotypes. (A) Cell suspensions of each strain were prepared by serial dilution and applied to YPD agar and incubated at 30 or 42°C or to YPD agar supplemented with 1.5 M NaCl, 5 mM caffeine, 10 nM rapamycin, 200 μM Congo red, or 0.05% SDS or at a pH of 8 and incubated at 30°C. (B) To determine resistance to nitrogen starvation, each strain was incubated in SD-N medium. Cell viability was determined as CFU counts from samples taken at intervals and expressed as a percentage of CFU at time zero. Data shown are the means and standard deviations of results of three independent experiments.