Fig. 5.

Overexpression of UME6 does not restore normal hyphal elongation to the aps3Δ/Δ vps21Δ/Δ mutant. UME6 was overexpressed from the ACT1 promoter in CAI4 (“wild-type” [WT] control), aps3Δ/Δ, vps21Δ/Δ, and aps3Δ/Δ vps21Δ/Δ strain backgrounds. Each strain was also transformed with the pACT1 vector alone (shown only for the control strain). (A) Strains were streaked to YPD and grown at 30°C. (B) Each strain was grown in YPD broth at 30°C, stained with FM4-64, and observed using a deconvolution microscope. Scale bar, 10 μM.