Table 1.
Oligonucleotides used in this study
| Primer | Sequence (5′→3′)a |
|---|---|
| APS3DISF | AATGATGGTCTTCCGAGACTAATGAAATTCTATACCAAAGTCGATATT |
| CCAACACAGAAATTGCTCTTGCTGTGGAATTGTGAGCGGATA | |
| APS3DISR | TATACTATTTTTGCATAATTCATTTCGTATTTATCGCTTTTACCTACCC |
| CAGAACCCTCTATCTCGAGACTTTCCCAGTCACGACGTT | |
| APS3AMPF2 | TCATCAGAGCTCCTTTGCACCAACAATGGGGGC |
| APS3AMPR | TCATCAGGATCCAGGAAGCAGAAAGGTTGATGC |
| APS3PBR | TGCCAGCCAAATACCAAATCC |
| APS3DETF | AAGATTTGGATGATATCAAGG |
| APS3DETR | CCTACCCCAGAACCCTCTATCTCG |
| UME6ORFF | TCATCAGTCGACGTATAAATGATTACCCATATGG |
| UME6AMPMLUR | TCATCAACGCGTTACGAGAATATTAATGTGTGC |
| ACT1PF | TCATCAGGTACCCCAGCCTCGTTTATAAACTTAGTC |
| ACT1PR | TCATCAGTCGACTTTGAATGATTATATTTTTTTAATATTAATATCGAG |
| MLT1GFPF | ACCTCAAAACTTGTTGAAGAACAAAGACAGTATTTTCTACTCTCTTG |
| CCAAAGAAGGTGGATACATAGATGGTGGTGGTTCTAAAGGTGAAGA | |
| ATTATT | |
| MLT1GFPR | TGTAAACTAAAAAAAATATTATTGTATAAATAAAAAAATCACTATAT |
| GAATATATATCGCACCGATATATATCTAGAAGGACCACCTTTGATT | |
| MLT1DETF | GATCTTAGTGTTAGATAGTGG |
| ARG4DETF | ATCAATTAACACAGAGATACC |
| ARG4DETR | CCGAGCTTGGCGTAATCATGG |
| HIS1F1268 | CCGCTACTGTCTCTACTTTG |
| HIS1DETF | TCATCCTCCAGGATCCCGCGG |
| URA3INTF | TTAGTGTTACGAATCAATGGC |
| URA3INTR | CAATTATAAATGTGAAGGGGG |
| GFPDETR | CATCACCTTCACCTTCACCGG |
a
Engineered restriction enzyme sites are underlined.