FIG. 3.

Bacteria lacking a T3SS translocon are cleared less rapidly from the lung. C57BL/6 mice were infected intranasally with 5 × 10⁴ CFU of ΔUT or pcrV:Tn5 bacteria and then sacrificed at 12 h.p.i.; 4-h.p.i. data are reproduced from Fig. 1 for comparison. (A) Bacterial recovery from lung tissue expressed as output (CFU per lung) over input (CFU per inoculum); lines indicate geometric means. (B) Total cells recovered in BAL fluid. Each point represents the value for an individual animal. P values for pairwise comparisons are indicated (Mann-Whitney test; n.s., not significant). The 12-h.p.i. data are from 2 independent experiments with 5 to 6 mice per group.