FIG. 3.

Expression of heterologous proteins in L. lactis or S. cerevisiae. Lactococcal cells were incubated with antibodies to SspB, and antibody reactivity was detected with FITC-labeled anti-rabbit secondary antibody. (A and C) Light microscopy. (B and D) Fluorescence microscopy. (A and B) L. lactis MG1363 (control) cells nonreactive with antibody. (C and D) L. lactis expressing SspB. (E) Western blots of lyticase-extracted fungal cell wall proteins reacted with monoclonal antibody to Als3p and antibody binding detected with HRP-linked anti-mouse secondary antibody. Lane 1, S. cerevisiae wild type; lane 2, S. cerevisiae expressing Als3p; lane 3, C. albicans NGY152 wild type; lane 4, C. albicans als3Δ/als3Δ.