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. 2010 Aug 30;78(11):4644–4652. doi: 10.1128/IAI.00685-10

FIG. 5.

FIG. 5.

Coaggregation between bacterial strains and S. cerevisiae expressing Als3p. FITC-labeled streptococci or lactococci were incubated with S. cerevisiae S150-2B parent cells or with cells constitutively expressing Als3p. Coaggregates were visualized by light or fluorescence microscopy. (A and B) S. cerevisiae S150-2B control cells mixed with L. lactis expressing SspB. (C and D) S. cerevisiae S150-2B expressing Als3p mixed with L. lactis expressing SspB, showing coaggregation. The arrows indicate yeast cells with or without adhered bacteria. (E) Percentages of S. cerevisiae parent (WT) or Als3p-expressing cells interacting with S. gordonii DL1 (Sg WT) or S. gordonii Δ(sspA sspB) [Sg Δ(sspAB)]. (F) Percentages of S. cerevisiae parent (WT) or Als3p-expressing cells interacting with L. lactis MG1363 control cells (Ll control) or L. lactis MG1363 expressing SspB (Ll SspB). The data are means and SD from 2 independent experiments (*, P < 0.05).