TABLE 3.
PCR analysis of OI-57 in STEC strains belonging to seropathotypes (SPT) A and Ba
| ORF | Gene product | No. of positive strains (total no. tested) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| SPT A (O157) (9) | SPT B |
SPT C |
SPT D/E (various O groups) (7) | ECOR (27) | |||||||
| O26 (9) | O111 (10) | O145 (7) | O103 (4) | O121 (2) | O113 (9) | O91 (4) | |||||
| Z2053 | AdfO | 9 | 9 | 9 | 7 | 2 | 2 | 0 | 0 | 0 | 0 |
| Z2054 | Ckf | 9 | 9 | 9 | 7 | 2 | 2 | 0 | 0 | 0 | 0 |
| Z2066 | Hypothetical protein | 9 | 9 | 10 | 7 | 4 | 2 | 0 | 0 | 0 | 7 |
| Z2096 | Hypothetical protein | 7 | 9 | 10 | 7 | 4 | 2 | 0 | 0 | 0 | 0 |
| Z2104 | Putative ARAC-type regulatory protein | 9 | 9 | 10 | 7 | 4 | 2 | 0 | 0 | 0 | 2 |
| Z2105 | Hypothetical protein | 7 | 9 | 10 | 7 | 4 | 2 | 2 | 0 | 0 | 2 |
| Z2148 | Hypothetical protein | 9 | 9 | 10 | 7 | 4 | 2 | 0 | 0 | 0 | 0 |
| Z2150 | Hypothetical protein | 9 | 9 | 8 | 1 | 3 | 2 | 0 | 0 | 0 | 0 |
a
The target genes represented eight ORFs scattered along the entire OI-57 genomic island. STEC strains belonging to SPTs C and D/E and nonpathogenic E. coli strains from the ECOR collection were also examined. The number of strains tested is reported in parentheses at the corresponding serogroup. The nomenclature of the ORFs refers to the sequence of STEC O157 strain EDL933 (GenBank accession no. AE005174).