FIG. 6.

NOD2 induces NF-κB activation in response to B. hermsii. Human embryonic kidney (HEK293) cells expressing (A) murine NOD1 or (B) murine NOD2 and luciferase under NF-κB promoter control were stimulated for 24 h with increasing concentrations of B. hermsii sonicate (0.8 to 25%; 0.5% B. hermsii sonicate corresponds to 1.25 × 10⁴ spirochetes) or specific control agonists (E. coli peptidoglycan [PGN]; 0.16 to 50 μg/ml). Luciferase assays were performed to determine NF-κB activation relative to mock-stimulated controls, and all values were normalized for transfection efficiency.