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. 2010 Aug 16;78(11):4691–4696. doi: 10.1128/IAI.00801-10

FIG. 2.

FIG. 2.

Quantitative analysis of SubAB-induced apoptosis in wt, bax−/−/bak−/−, and bim−/−/puma−/− MEFs. (A) In situ labeling of phosphatidylserine on the surface of apoptotic cells. Cells were treated with either SubAB (100 ng/ml) or SubAA272B (100 ng/ml) for 24 h. Phosphatidylserine was labeled with annexin V conjugated to Alexa 488 dye. (B) In situ TUNEL of DNA fragmentation in MEFs that were treated with either SubAB (100 ng/ml) or SubAA272B (100 ng/ml) for 30 h. Data represent the means ± SDs from two independent experiments. *, P < 0.05, Student's unpaired, two-tailed t test.