TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| H. ducreyi strains | ||
| 35000HP | Human-passaged H. ducreyi strain | 57 |
| 35000HPΔcpxA | 35000HP ΔcpxA::Cmr | This study |
| 35000HPΔcpxR | 35000HP ΔcpxR::Cmr | 35 |
| E. coli strains | ||
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169recA1endA1hsdR17(rK− mK+) phoAsupE44 λ−thi-1gyrA96relA1 | Invitrogen |
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araD139Δ(ara-leu)7697galUgalKrpsLendA1nupG | Invitrogen |
| HB101 | F−thi-1hsdS20(rB− mB−) supE44recA13ara-14leuB6proA2lacY1galK2rpsL20 (Strr) xyl-5mtl-1 | Promega |
| Plasmids | ||
| pCR2.1 | E. coli cloning vector; Ampr | Invitrogen |
| pML117 | pCR2.1 containing a 35000HP cpxA gene with a 1.15-kb deletion | This study |
| pML118C | pML117 with a cat cartridge in the deletion site in cpxA | This study |
| pLS88 | H. ducreyi shuttle vector; Kmr | 66 |
| pML141 | pLS88 containing the wild-type 35000HP cpxA gene | This study |
| pML153 | pL141 with the H253Q substitution in CpxA | This study |
| pML125 | pLS88 containing the wild-type 35000HP cpxR gene | 35 |
| pML154 | pML125 with the D52A substitution in CpxR | This study |